yitI [2018-11-21 15:16:49]

similar to glyphosate N-acetyltransferase

locus: BSU11000

pI: 9.17

mw: 17.47 kDa

protein length: 154 aa

gene length: 462 bp

function: unknown

product: unknown

essential: no

ec: 2.3.1.-

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,178,218 → 1,178,682

The protein

Protein family

• N-acetyltransferase domain (according to Swiss-Prot)

Structure

• [PDB|2JDC] (from B. licheniformis, 59% identity) [pubmed|17272278]

Expression and Regulation

Operons

• genes: [gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]-[gene|1A31885756344F8BD2BED4E4526D43D6D5AB85F3|yitH]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B205 (yitI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1204 NBRP B. subtilis, Japan]
• BKE11000 (Δ[gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGTTTTTCTATATC, downstream forward: _UP4_TGATCTTATGGACGTAGTAG
• BKK11000 (Δ[gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGTTTTTCTATATC, downstream forward: _UP4_TGATCTTATGGACGTAGTAG

References

• 19258532,17272278